![]() ![]() ![]() ![]() In human colonic PCmsrc cells transformed by c- src oncogene, the hepatocyte growth factor (HGF)-dependent invasiveness of collagen gels was abolished by 0.1 μ m PAF and restored by the PAF-R antagonists WEB2086 and SR27417. An elongated splice variant was identified in the 5′-untranslated region of the tissue-type PAF-R transcript (334 bp) in colonic epithelial crypts and tumors. PAF-R gene transcripts were analyzed by reverse transcription-polymerase chain reaction and Southern blot, using three sets of primers corresponding either to the coding region of the human PAF-R sequence (polymerase chain reaction product: 682 base pairs (bp)) or to the leukocyte- and tissue-type transcripts of 166 and 252 bp, respectively. This study was designed to characterize platelet-activating factor receptor (PAF-R) expression and function in normal and cancerous human colonic epithelial cells. ![]()
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